THC/Marijuana fights liver cancer

First we learned that the cannabinoids produced by the cannabis plant fight and kill cancer cells now we are beginning to uncover the mechanisms by which cannabinoids target and kill cancer cells. Alcohol promotes cancer while marijuana suppresses cancer, what's wrong with this picture? I could go buy gallons and gallons of booze legally, but the government will destroy your life if you market marijuana. Cell Death Dis. 2013 May 2;4:e618. doi: 10.1038/cddis.2013.141.Involvement of PPARγ in the antitumoral action of cannabinoids on hepatocellular carcinoma.Vara D, Morell C, Rodríguez-Henche N, Diaz-Laviada I.SourceBiochemistry and Molecular Biology Unit, Department of System Biology, School of Medicine, University ofAlcala, 28871 Madrid, Spain.AbstractCannabinoids exert antiproliferative effects in a wide range of tumoral cells, including hepatocellular carcinoma HCC cells. In this study, we examined whether the PPARγ-activated pathway contributed to the antitumor effect of two cannabinoids, Δ9-tetrahydrocannabinol THC and JWH-015, against HepG2 and HUH-7 HCC cells. Both cannabinoids increased the activity and intracellular level of PPARγ mRNA and protein, which was abolished by the PPARγ inhibitor GW9662. Moreover, genetic ablation with small interfering RNA siRNA, as well as pharmacological inhibition of PPARγ decreased the cannabinoid-induced cell death and apoptosis. Likewise, GW9662 totally blocked the antitumoral action of cannabinoids in xenograft-induced HCC tumors in mice. In addition, PPARγ knockdown with siRNA caused accumulation of the autophagy markers LC3-II and p62, suggesting that PPARγ is necessary for the autophagy flux promoted by cannabinoids. Interestingly, downregulation of the endoplasmic reticulum stress-related protein tribbles homolog 3 TRIB3 markedly reduced PPARγ expression and induced p62 accumulation, which was counteracted by overexpression of PPARγ in TRIB3-knocked down cells. Taken together, we demonstrate for the first time that the antiproliferative action of the cannabinoids THC and JWH-015 on HCC, in vitro and in vivo, are modulated by upregulation of PPARγ-dependent pathways.

via Involvement of PPARγ in the antitumoral actio... [Cell Death Dis. 2013] - PubMed - NCBI.

CANNABIDIOL INHIBITS ANGIOGENESIS BY MULTIPLE... [Br J Pharmacol. 2012] - PubMed - NCBI

Again we see the amazing anti-tumor effects of the unique compounds produced by the marijuana/cannabis plant. Again we ask, why is growing, using or distributing this plant illegal? According to the researchers "CBD could represent a potential effective agent in cancer therapy" CANNABIDIOL INHIBITS ANGIOGENESIS BY MULTIPLE MECHANISMS.

Solinas M, Massi P, Cantelmo A, Cattaneo M, Cammarota R, Bartolini D, Cinquina V, Valenti M, Vicentini L, Noonan D, Albini A, Parolaro D.

Source

Department of Biomedical, Computer and Communication Sciences, University of Insubria, Busto Arsizio (VA), Italy, Department of Pharmacology, Chemotherapy and Toxicology, University of Milan, 20129 Milan, Italy, Oncology Research Laboratory, Science and Technology Park, IRCCS MultiMedica, 20138 Milan, Italy, Department of Biotechnology and Life Sciences, University of Insubria, 21100 Varese, Italy.

Abstract

Background and purpose: Several studies demonstrated anti-proliferative and pro-apoptotic actions of cannabinoids on several tumours, together with their anti-angiogenic properties. The non-psychoactive cannabinoid cannabidiol (CBD) effectively inhibits in vitro and in vivo the growth of different types of tumours and down-regulates some pro-angiogenic signal produced by glioma cells. As its anti-angiogenic properties have not been thoroughly investigated to date, and given its very favorable pharmacological and toxicological profile, here we evaluated CBD ability to modulate tumour angiogenesis. Experimental approach: We firstly evaluated CBD effect on human umbilical vein endothelial cell (HUVEC) proliferation and viability- through MTT assay and FACS analysis-and in vitro motility-both in a classical Boyden chamber test and in a wound-healing assay. We next investigated CBD effects on different angiogenesis-related proteins released by HUVECs, using an angiogenic Array Kit and an enzyme-linked immunosorbent assay (ELISA) directed at MMP2. Afterwards we evaluated in vitro angiogenesis in treated HUVECs invading a Matrigel layer and in HUVEC spheroids embedded into collagen gels. We further characterized CBD effects using a Matrigel sponge model of in vivo angiogenesis. Key results: CBD induced HUVEC cytostasis without inducing apoptosis, inhibited HUVEC migration, invasion, and sprouting in vitro, and angiogenesis in vivo in matrigel sponges. These effects were associated with down-modulation of several angiogenesis-related molecules. Conclusions and Implications: This study reveals that CBD inhibits angiogenesis by multiple mechanisms. Its dual effect on both tumour and endothelial cells reinforces the hypothesis that CBD could represent a potential effective agent in cancer therapy. © 2012 The Authors. British Journal of Pharmacology © 2012 The British Pharmacological Society.

© 2012 The Authors. British Journal of Pharmacology © 2012 The British Pharmacological Society.

via CANNABIDIOL INHIBITS ANGIOGENESIS BY MULTIPLE... [Br J Pharmacol. 2012] - PubMed - NCBI.

CANNABIDIOL INHIBITS ANGIOGENESIS BY MULTIPLE... [Br J Pharmacol. 2012] - PubMed - NCBI

Again we see the amazing anti-tumor effects of the unique compounds produced by the marijuana/cannabis plant. Again we ask, why is growing, using or distributing this plant illegal? According to the researchers "CBD could represent a potential effective agent in cancer therapy" CANNABIDIOL INHIBITS ANGIOGENESIS BY MULTIPLE MECHANISMS.

Solinas M, Massi P, Cantelmo A, Cattaneo M, Cammarota R, Bartolini D, Cinquina V, Valenti M, Vicentini L, Noonan D, Albini A, Parolaro D.

Source

Department of Biomedical, Computer and Communication Sciences, University of Insubria, Busto Arsizio (VA), Italy, Department of Pharmacology, Chemotherapy and Toxicology, University of Milan, 20129 Milan, Italy, Oncology Research Laboratory, Science and Technology Park, IRCCS MultiMedica, 20138 Milan, Italy, Department of Biotechnology and Life Sciences, University of Insubria, 21100 Varese, Italy.

Abstract

Background and purpose: Several studies demonstrated anti-proliferative and pro-apoptotic actions of cannabinoids on several tumours, together with their anti-angiogenic properties. The non-psychoactive cannabinoid cannabidiol (CBD) effectively inhibits in vitro and in vivo the growth of different types of tumours and down-regulates some pro-angiogenic signal produced by glioma cells. As its anti-angiogenic properties have not been thoroughly investigated to date, and given its very favorable pharmacological and toxicological profile, here we evaluated CBD ability to modulate tumour angiogenesis. Experimental approach: We firstly evaluated CBD effect on human umbilical vein endothelial cell (HUVEC) proliferation and viability- through MTT assay and FACS analysis-and in vitro motility-both in a classical Boyden chamber test and in a wound-healing assay. We next investigated CBD effects on different angiogenesis-related proteins released by HUVECs, using an angiogenic Array Kit and an enzyme-linked immunosorbent assay (ELISA) directed at MMP2. Afterwards we evaluated in vitro angiogenesis in treated HUVECs invading a Matrigel layer and in HUVEC spheroids embedded into collagen gels. We further characterized CBD effects using a Matrigel sponge model of in vivo angiogenesis. Key results: CBD induced HUVEC cytostasis without inducing apoptosis, inhibited HUVEC migration, invasion, and sprouting in vitro, and angiogenesis in vivo in matrigel sponges. These effects were associated with down-modulation of several angiogenesis-related molecules. Conclusions and Implications: This study reveals that CBD inhibits angiogenesis by multiple mechanisms. Its dual effect on both tumour and endothelial cells reinforces the hypothesis that CBD could represent a potential effective agent in cancer therapy. © 2012 The Authors. British Journal of Pharmacology © 2012 The British Pharmacological Society.

© 2012 The Authors. British Journal of Pharmacology © 2012 The British Pharmacological Society.

via CANNABIDIOL INHIBITS ANGIOGENESIS BY MULTIPLE... [Br J Pharmacol. 2012] - PubMed - NCBI.